Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 protein (GTX01548-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) protein (GTX01548-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Inhibition ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro) (coated at 2 μg/mL) inhibiting by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL), and binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SDS-PAGE of GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active).

Functional ELISA analysis of immobilized GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active) at 0.5μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 0.06-0.25μg/mL.