Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

1μg of GTX135684-pro SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.