Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135386) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (1 μg, GTX135684-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135386 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 μg/mL to 2 μg/mL. The coated protein is detected with (GTX135386) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.