Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

SARS-CoV-2 nsp12 detects SARS-CoV-2 nsp12 protein at cytoplasm by immunohistochemical analysis.
Sample: human SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 nsp12 stained by SARS-CoV-2 nsp12 (GTX636916) diluted at 1:255.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min