Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp14 antibody [HL2539] (GTX638908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nsp14 antibody [HL2539] detects SARS-CoV-2 (COVID-19) nsp14 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: SARS-CoV-2 (COVID-19) nsp14 stained by SARS-CoV-2 (COVID-19) nsp14 antibody [HL2539] (GTX638908) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).