Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2709] (GTX639463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2709] detects SARS-CoV-2 (COVID-19) nsp3 / PL-pro protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp3 / PL-pro stained by SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2709] (GTX639463) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).