Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (GTX636719) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm by immunohistochemical analysis.
Sample: SARS-CoV-2 (COVID-19) nsp7 transfected 293T cell FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (GTX636719) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (GTX636719) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.