Recommended antibody pairs for sandwich ELISA : Capture: GTX635685, Detection: GTX635712 / GTX635688 / GTX635678. or Capture: GTX635679 / GTX635689, Detection: GTX635686-01. Please notice that detection antibodies need to be conjugated to HRP when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.
Recommended antibody pairs for Lateral Flow : Capture: GTX635688, Detection: GTX635686 / GTX637789 Please notice that the detection antibodies need to be conjugated to Gold to function when paired with capture antibodies. Please contact us for custom Gold-conjugated antibody.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His tagged full length protein of YP_009724397.2
Expression System
HEK293 cells
Purity
>90%
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.Sentivity : 2.68 pg/mL
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.Sensitivity : 39.06 pg/mLPlease notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635808. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX135592-pro Lateral Flow Image
Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control. Capture: S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])Detection:S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])Samples (40 ng) : 1. Lysis buffer2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL).
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX135592-pro Lateral Flow Image
Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control. Pair for S : Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688) Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689) Pair for A: Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789) Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) Pair for B : Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) Samples (40 ng) : 1. Lysis buffer 2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)
Show More +
GTX135592-pro ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) as detection antibody at concentration of 1 μg/mL.
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GTX135592-pro WB Image
SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX135592-pro Image
0.5 μg of GTX135592-pro SARS-CoV-2 (COVID-19) nucleocapsid protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
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