SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3)

Cat. No. GTX537371-23
Cat. No.  GTX537371-23
SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3). GTX537371-23
GTX537371-23 PLA Image

Detection of SARS-CoV-2 (COVID-19) membrane protein in a transfected HEK293T cell FFPE Cell Pellet Block (GTX435640) section using the SARS-CoV-2 Membrane Protein Proximity Ligation Assay Kit (Cy3) (GTX537371-23). PLA signals appear yellow and nuclei are blue.
A) Positive PLA reaction in transfected HEK293T cells.
B) Negative control with mock-transfected HEK293T cells.
C) Negative control using one non-specific PLA antibody probe in transfected HEK293T cells.

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SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3). GTX537371-23
GTX537371-23 Image
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SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3). GTX537371-23
SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3). GTX537371-23
  • Applications
    PLA
  • Reactivity
    SARS Coronavirus 2
Summary
The SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3) enables the detection of SARS-CoV-2 membrane protein in virus-infected tissue or infected/transfected cell block samples. The viral membrane protein is detected using two oligonucleotide-conjugated primary antibodies, called PLA probes. Only when both PLA probes bind to the membrane protein can the assay generate an amplified signal. This provides researchers with a highly specific and sensitive diagnostic tool.
Content

Item Name Quantity Storage
A1 Blocking Solution, 10 mL 2 bottles -20 ºC
A2 Antibody (Ab)-Oligo A, 40 µL 1 tube -20 ºC
A3 Antibody (Ab)-Oligo B, 40 µL 1 tube -20 ºC
A4 Ligase (400 U/µL), 10 µL 1 tube -20 ºC
A5 5x Ligation Solution, 500 µL 1 tube -20 ºC
A6 Polymerase (10 U/µL), 10 µL 1 tube -20 ºC
A7 5x Amplification Solution, 500 µL 1 tube -20 ºC
A8 Detection Probe (Cy3), 50 µL 1 tube -20 ºC
B1 10x Probe Solution, 200 µL 1 tube 4°C or below
B2 20x Wash Buffer, 50 mL 2 bottles 4°C or below

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