Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a stained by SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

WB analysis of SARS-CoV-infected Vero E6 cells harvested at different time points postinfection using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

ICC/IF analysis of Vero E6 cells expressing ORF7a using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV ORF7a protein by western blot analysis. Non-transfected (-) and SARS-CoV ORF7a-transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.

The data was published in the 2020 in Cell Death Dis. PMID: 32814759