Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1(GTX103371) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: NCI-H929
7.5% SDS PAGE
GTX103371 diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX103371 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SART1(GTX103371) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min