Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684)
5 % SDS-PAGE
The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SART3 (GTX107684) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SART3(GTX107684) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.