Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SBDS antibody [N1C3] (GTX109168) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX109168 diluted at 1:1000

SBDS antibody [N1C3] detects SBDS protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SBDS antibody [N1C3] (GTX109168) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: H1299
B: HeLa
C: Hep G2 (GTX27900)
12% SDS PAGE
GTX109168 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using SBDS(GTX109168) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using SBDS(GTX109168) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min