Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SCMH1 antibody (GTX115653) diluted at 1:1000.

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX115653 diluted at 1:10000

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115653 diluted at 1:5000