Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SCRIB antibody [HL1639] (GTX637220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SCRIB antibody [HL1639] detects SCRIB protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
SCRIB stained by SCRIB antibody [HL1639] (GTX637220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min