Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: MitoTracker® Red CMXRos, a mitochondria marker.
Blue: Fluoroshield with DAPI (GTX30920).

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.