WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates (in NETN lysis buffer) using GTX23625 SERCA2 ATPase antibody.
Loading : 50μg
Dilution : 1 μg/ml

IP analysis of 293T cell lysate using GTX23625 SERCA2 ATPase antibody.
IP protein : 0.5 -1.0 mg
Dilution : 1 μg/ml for WB ; 0.5-1 mg lysate / 6μg antibody for IP reaction
Loading: 20% of IP loaded