Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using SERPINE2(GTX124069) antibody at 1:500 dilution.

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX124069 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SERPINE2(GTX124069) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.