Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN1 antibody [HL1750] (GTX637400) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SESN1 antibody [HL1750] detects SESN1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SESN1 stained by SESN1 antibody [HL1750] (GTX637400) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min