HeLa whole cell and nuclear extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.

SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N1], N-term (GTX115305)
5 % SDS-PAGE
The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.