Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p53P2.

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N2C1], Internal (GTX110219)
5 % SDS-PAGE
The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]