ICC/IF analysis of U2OS cells using GTX03696 SGK1 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2 μg/mL

IHC-P analysis of human rectal cancer tissue using GTX03696 SGK1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)

IHC-P analysis of human rectal cancer tissue using GTX03696 SGK1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)

FACS analysis of SiHa cells using GTX03696 SGK1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

WB analysis of various cell lysates using GTX03696 SGK1 antibody.
Dilution : 0.5 μg/mL
Loading : 50 μg per lane