Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SHANK1 antibody [C3], C-term detects SHANK1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SHANK1 stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SHANK1 antibody [C3], C-term detects SHANK1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SHANK1 antibody [C3], C-term detects SHANK1 protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Blue: Hoechst 33342 staining.

SHANK1 antibody [C3] detects SHANK1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: SHANK1 antibody [C3] (GTX107701) diluted at 1:250. Blue: DAPI

SHANK1 antibody [C3], C-term detects SHANK1 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SHANK1(GTX107701) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min