Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody (GTX101062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101062 diluted at 1:500

SHP2 antibody detects PTPN11 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SHP2 antibody (GTX101062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using SHP2(GTX101062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SHP2 antibody detects PTPN11 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
SHP2 antibody (GTX101062) dilution: 1:1000