Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2274] (GTX638326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SIGMAR1 antibody [HL2274] detects SIGMAR1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SIGMAR1 stained by SIGMAR1 antibody [HL2274] (GTX638326) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min