Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] (GTX115389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SIGMAR1(GTX115389) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) andSIGMAR1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] (GTX115389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] (GTX115389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.