APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human SIRP alpha Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human SIRP alpha. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
signal regulatory protein alpha , BIT , CD172A , MFR , MYD-1 , P84 , PTPNS1 , SHPS1 , SIRP
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659](/templates/default/js/lightbox/images/loading.gif "Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659")
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GTX640659 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SIRP alpha antibody [HL3149] (GTX640659) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for SIRP alpha antibody [HL3149] (GTX640659). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SIRP alpha antibody [HL3149] (GTX640659). Be the first to share your experience with this product.
![Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659](/upload/website/prouct_img/small/GTX640659/GTX640659_T-45481_20240802_WB_TPM_watermark_24080602_657.webp "Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659")
![Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659](/upload/website/prouct_img/normal/GTX640659/GTX640659_T-45481_20240802_WB_TPM_watermark_24080602_657.webp "Anti-SIRP alpha antibody [HL3149] used in Western Blot (WB). GTX640659")