Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SIRT3 antibody [C3], C-term detects SIRT3 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SIRT3(GTX106292) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of methanol-fixed A549, using SIRT3(GTX106292) antibody at 1:200 dilution.

SIRT3 antibody detects SIRT3 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody (GTX106292) at a dilution of 1:1000.