Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
GTX106675 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX106675 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SKP1 antibody (GTX106675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SKP1 antibody detects SKP1 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SKP1 antibody (GTX106675) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min