Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SLC25A13(GTX109001) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:1000 and competitor's antibody (10789-1-AP) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A13 protein stained by SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX109001 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Wild-type (WT) and SLC25A13 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min