SMAD2 / SMAD3 antibody immunoprecipitates SMAD2 / SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 / SMAD3 antibody (GTX111123) The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 μg of SMAD2 / SMAD3 antibody (GTX111123).
Western blot analysis was performed using SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
SMAD2 / SMAD3 antibody (GTX111123) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2 / SMAD3 (GTX111123) antibody at 1:200 dilution.

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

The data was published in the 2022 in Front Pharmacol. PMID: 35517780