Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX111075 diluted at 1:500

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody (GTX111075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD2(GTX111075) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SMAD2 antibody detects SMAD2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SMAD2 antibody (GTX111075) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SMAD2 antibody detects SMAD2 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
SMAD2 antibody (GTX111075) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal PLoS Genet in 2019. PMID: 31306413