Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX110402 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [C2C3], C-term (GTX110402) or SMC3 antibody [N1N2], N-term (GTX109827).
Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [C2C3], C-term (GTX110402).
Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX110402 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SMC3(GTX110402) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX110402 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMC3(GTX110402) antibody at 1:200 dilution.