Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC6L1 antibody [N3C2], Internal (GTX116832) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SMC6L1 antibody [N3C2], Internal detects SMC6L1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SMC6L1 stained by SMC6L1 antibody [N3C2], Internal (GTX116832) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using SMC6L1(GTX116832) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SMC6L1 antibody [N3C2], Internal detects SMC6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMC6 protein stained by SMC6L1 antibody [N3C2], Internal (GTX116832) diluted at 1:500.
Blue: Hoechst 33342 staining.