Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SMNDC1 antibody [N1C3-2] (GTX118263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMNDC1(GTX118263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SMNDC1 antibody [N1C3-2] detects SMNDC1 protein at nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SMNDC1 antibody [N1C3-2] (GTX118263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using SMNDC1(GTX118263) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using SMNDC1(GTX118263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min