Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD2 antibody (GTX116510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using SMYD2(GTX116510) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
B: HCT116
10% SDS PAGE
GTX116510 diluted at 1:1000

Immunoprecipitation of SMYD2 protein from HeLa whole cell extracts using 5 μg of SMYD2 antibody (GTX116510).
Western blot analysis was performed using SMYD2 antibody (GTX116510).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.