Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.

SMYD3 antibody immunoprecipitates SMYD3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 μg of SMYD3 antibody (GTX121956) 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 antibody (GTX121956) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMYD3(GTX121956) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121956) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.