Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX125918) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SNAI1 antibody detects SNAI1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SNAI1 antibody (GTX125918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 protein stained by SNAI1 antibody (GTX125918) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Oncotarget in 2016.PMID: 26840566

The data was published in the journal Oncotarget in 2016.PMID: 26840566