Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SOCS1(GTX100657) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SOCS1 antibody detects SOCS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOCS1 protein stained by SOCS1 antibody (GTX100657) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100657 diluted at 1:1000