Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with SOCS3 antibody [N1C3] (GTX113049) diluted at 1:10000 and competitor's antibody (#52113) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) Raw264.7 whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOCS3 antibody [N1C3] (GTX113049) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.