IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount :
MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.

ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail.
Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4ºC)

IP analysis of HEK293 cells cultured in the presence and absence of 10μM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation.
Antibody amount : 1-5 μg