Dotblot analysis of anti-SOD2 (Acetyl Lys68) antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) varied dilution.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.