Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX103230) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SOD2 antibody (GTX103230) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish liver
12% SDS PAGE
GTX103230 diluted at 1:1000