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SOX2 antibody [HL1193]

Recombinant antibody KO/KD Comparison
Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505
Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505
Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505
Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505
Anti-SOX2 antibody [HL1193] used in IHC (Paraffin sections) (IHC-P). GTX636505
Anti-SOX2 antibody [HL1193] used in IHC (Paraffin sections) (IHC-P). GTX636505
Anti-SOX2 antibody [HL1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636505
Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

Cat. No. GTX636505

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1193

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)
See all SOX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SOX2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

GTX636505 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

GTX636505 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

GTX636505 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

GTX636505 WB Image

Mouse ESC extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOX2 antibody [HL1193] used in IHC (Paraffin sections) (IHC-P). GTX636505

GTX636505 IHC-P Image

SOX2 antibody detects SOX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX2 antibody [HL1193] used in IHC (Paraffin sections) (IHC-P). GTX636505

GTX636505 IHC-P Image

SOX2 antibody detects SOX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOX2 antibody [HL1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636505

GTX636505 ICC/IF Image

SOX2 antibody [HL1193] detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 stained by SOX2 antibody [HL1193] (GTX636505) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-SOX2 antibody [HL1193] used in Western Blot (WB). GTX636505

GTX636505 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations