Non-transfected (–) and transfected (+) NT2D1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SOX2 antibody [HL1193] detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 stained by SOX2 antibody [HL1193] (GTX636505) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

SOX2 antibody detects SOX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SOX2 antibody detects SOX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse ESC extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.