SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SOX3 antibody (GTX129235) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of SOX3 protein from NT2D1 whole cell extracts using 5 μg of SOX3 antibody (GTX129235).
Western blot analysis was performed using SOX3 antibody (GTX129235) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX3 antibody (GTX129235) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SOX3 antibody detects SOX3 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: SOX3 antibody (GTX129235) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SOX3 antibody (GTX129235) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SOX3 antibody detects SOX3 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX3 protein stained by SOX3 antibody (GTX129235) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.