Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min