SPR antibody [N2C3] detects SPR protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SPR antibody [N2C3] (GTX113552) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX113552 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using SPR(GTX113552) antibody at 1:200 dilution.

SPR antibody [N2C3] immunoprecipitates SPR protein in IP experiments.
IP samples: A431 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SPR protein by 4 μg SPR antibody [N2C3] (GTX113552)
5 % SDS-PAGE
The immunoprecipitated SPR protein was detected by SPR antibody [N2C3] (GTX113552) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]