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SQSTM1 / P62 antibody [HL1113]

Recombinant antibody Comparison Orthogonal
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

Cat. No. GTX636328

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1113

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SQSTM1 / P62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909970

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm, cytosol,Lysosome,Nucleus,Endoplasmic reticulum

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328

GTX636328 ICC/IF Image

Orthogonal

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328

GTX636328 IHC-P Image

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang Y et al. Front Endocrinol (Lausanne) 2022; 13 : 1051374 Calcitriol-enhanced autophagy in gingival epithelium attenuates periodontal inflammation in rats with type 2 diabetes mellitus.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations