Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SQSTM1 antibody [N3C1], Internal (GTX100685) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [N3C1], Internal] (GTX100685) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal (GTX100685).
Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GTX100685).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
12% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Sci Rep in 2017. PMID: 28134285

The data was published in the journal Antioxidants (Basel) in 2019.PMID: 31906147

The data was published in the journal Antioxidants (Basel) in 2019.PMID: 31906147

The data was published in the journal Biomolecules in 2019.PMID: 31505885

The data was published in the journal Biomolecules in 2019.PMID: 31505885

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

The data was published in the 2020 in Int J Mol Sci. PMID: 33260618

The data was published in the journal Biomolecules in 2019.PMID: 31505885

The data was published in the journal PLoS One in 2015.PMID: 25946033