Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX111393) diluted at 1:7000.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX111393) diluted at 1:10000.

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
C: HL-60
D: NCI-H929
10% SDS PAGE
GTX111393 diluted at 1:10000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SQSTM1(GTX111393) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.